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注射器选择指南
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第一步:注射器设计方面



第二步选择针头类型

第三步推杆的密封导购


第四步或者联系本公司业务人员给你选型。
 
用PHERAstar Plus Homogeneous IgG AlphaLISA® 分析  
ALA-1/4T as a fluorescence analyser with isothermal amplification function Preliminary report  
1. 荧光偏振(Fluorescence Polarization )的应用文章,注明:以下Fluorescence Polarization 使用FP代替,FP原理请查看链接

1.01 在实时中使用荧光偏振发检测Ribozyme Reactions

K.K. Singh, T. Rücker, A. Hanne, R. Parwaresch and G. Krupp

Institut for Hematopathology, University of Kiel, Kiel, Germany

BioTechniques 29: 344-351 (August 2000)

1.02 均相高通量荧光筛选(HTRF):荧光偏振 和关联能谱法分析HTRF原理请查看链接

Keith J Moore, Stephen Ashman, Jonathan Saunders, Farid Khan, Sandra Turconi and Andrew J Pope

Molecular Interactions & New Assay Technologies, SmithKline Beecham, Harlow, Essex, UK.

(Available only as a hardcopy)

1.03 均相高通量荧光筛选: 药物-RNA的交叉作用

Sandra Turconi1, Victoria Emerick2, David T Davies1, Kelvin Nurse2, Lisa Hegg2, David G Tew2,

Eric Hunt1, Susannah Davies1, Kenneth GM Fantom1, Keith J Moore1 and Andrew J Pope1

SmithKline Beecham Pharmaceuticals,

1New Frontiers Science Park (North), Third Avenue, Harlow, Essex CM19 5AW, UK

2Upper Merion-West, 709 Swedeland Road, King of Prussia, PA 19406, USA

(Available only as a hardcopy)

1.04Characterization of the kinetics and thermodynamics of Escherichia coli Topoisomerase 1:DNA interactions using fluorescently-labelled substrates

N. Benson, K.J. Moore, S. Turconi, S. Ashman, J.P. Saunders and A.J. Pope

Molecular Interactions and New Assay Technologies, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park

(North), Third Avenue, Harlow, Essex CM19 5AW, UK.

(Available only as a hardcopy)

1.05 在高通量筛选中使用荧光偏振分析小分子抑制剂

Sean T Quinn1, Paul B. Taylor1, Jonathan Saunders2, Keith J. Moore2, David T. Davies2, Sandra Turconi2, Thomas Meek1, Neil Pearson2, Andrew J. Pope2, Georgi Chan1, Reuy Wang1 and Victoria L. Emerick1,

SmithKline Beecham Pharmaceuticals

1Upper Merion-West 709 Swedeland Road, King of Prussia, PA 19406, USA

2New Frontiers Science Park (North), Third Avenue, Harlow, Essex CM19 5AW, UK

(Available only as a hardcopy)

1.06 使用FP检测蛋白与蛋白之间的定量

David M. Jameson1 and Steven E. Seifried

Department of Genetics and Molecular Biology, University of Hawaii, 1960 Eas-West Road, Honolulu, Hawaii 96822

1.07 利用FP发检测蛋白酶的活性

Leanna M. Levine, * Marshall L. Michener, * Mihaly V. Toth, t and Barry C. Holwerda t

*Monsanto Corporate Research and tSearle Research and Development, 700 Chesterfield Parkway North, St. Louis, Missouri 63198

Analytical Biochemistry 247, 83-88 (1997)

1.08 在FP中改进分析功能且开发新的Fluor Chemis

*C.G. Norey, A.J. Harris, S. Cox, J.F. Wright, J.C. Ireson, M.J. Hatcher, S. Hardwicke, M. Briggs, N. Bosworth, P.M. Baxendale and D.D. Burns Nycomed Amersham plc, Cardiff Laboratories, Forest Farm, Whitchurch, Cardiff, CF14 7YT, Wales, U.K. Telephone: +44(0)29

2052 6439, fax: +44 (0) 29 20 52 6230, email: Christopher.norey@eu.apbiotech.com

1.09 FP(荧光偏振-一个新的细胞和分子生物的检测工具)

William J. Checovich, Randall E. Bolger and Thomas Burke

Nature 375, 254-256 (1995)

1.10 FKBP12荧光素配合剂可以做高通量FP的分析

Gene M. Dubowchik, Jonathan L. Ditta, John J. Herbst, Sagarika Bollini and Alexander Vinitsky

Bioorganic & Medicinal Chemistry 10, 559-562 (2000)

1.11 The application of fluorescence polarisation for the enhanced detection of green fluorescent protein (GFP) in the presence of cellular auto-fluorescence and other green fluorescent compounds

A. W. Knight, N. J. Goddard, P. R. Fielden, A. L. Gregson, N. Billinton, M. G. Barker and R. M. Walmsley

Analyst, 125, 499–506 (2000)

1.12 Bocillin FL,一个可以检测 Penicillin-Binding蛋白的高灵敏的试剂

Genshi Zhao, Timothy I. Meier, Steven D. Kahl, Kyle R. Gee, And Larry C. Blaszczak

Antimicrobial Agents and Chemotherapy 43(5), 1124-1128 (1999)

 1.13 荧光偏振检测普通的SNP基因

T.M. Hsu, X. Chen1, S. Duan, R.D. Miller, and P.-Y. Kwok

BioTechniques, 31(3), 560-570 (2001)

1.14 在均相核算荧光偏振的分析

Xiangning Chen, Leanna Levine and Pui-Yan Kwok

Genome Research 9, 492-498 (1999)

1.15 在荧光偏振中平衡常数分离的确定

Linda Prystay, Mylene Gosselin, and Peter Banks

Journal of Biomolecular Screening, 6(3), 141-150 (2001)

1.16 使用荧光偏振从区别绿色荧光蛋白

Fluorescence polarization discriminates green fluorescent protein from interfering autofluorescence in a micro plate assay for genotoxicity

Andrew W. Knight , Nicholas J. Goddard, Nicholas Billinton, Paul A. Cahill, Richard M. Walmsley

Journal of Biochemical and Biophysical Methods 51, 165-177 (2002)

1.17 新颖的荧光探针的合成做雌激素受体

Maciej Adamczyk, Rajarathnam E. Reddy and Zhiguang Yu

Bioorganic & Medicinal Chemistry Letters 12, 1283-1285 (2002)

1.18 SNP基因用FP检测 Pui-Yan Kwok

Human Mutation 19, 315-323 (2002)

1.19从抗菌素组合肽库中分离肽用小分子酶抑制剂检测

R Hyde-DeRuyscher, LA Paige, DJ Christensen, N Hyde-DeRuyscher, A Lim, ZL Fredericks, J Kranz, P Gallant, J Zhang, SM Rocklage, DM Fowlkes, PA Wendler and PT Hamilton

Chemistry & Biology 7(1), 17-25 (2000)

1.20 使用荧光检测工作场所气相过氧化氢

Jorg Meyer and Uwe Karst

Analytica Chimica Acta 401, 191-196 (1999)

 2. 荧光强度和荧光能量共振转移 (FRET)的应用FI,FRET的检测原理,请查看链接

2.01 在384-1536孔板中的酵母(Human Progesterone Receptor)中一个功能转录的测试

Michael Berg1, Karin Undisz1, Ralf Thiericke1, Thomas Moore2, and Clemens Posten3 1Hans-Knoell-Institut für Naturstoff-Forschung e.V., Jena, Germany

2CyBio Instruments GmbH, Jena Germany

3Institut für Mechanische Verfahrenstechnik und Mechanik der Universität Karlsruhe (TH), Karlsruhe, Germany

Journal of Biomolecular Screening 5, 71-76 (2000)

2.02 使用BMG FLUOstar 测试dsDNA PicoGreen DNA定量的荧光定量

Dr. Matthias Kuhn

ConGen Biotechnologie GmbH / Robert Rössle Straße 10 / 13125 Berlin

2.03利用FRET探针和equence specific primer-polymerase chain reaction (SSP-PCR)测试高通量class I HLA 基因

A.R. Geisthorpe, R.S. Wells, A.P. Lowe, S. Tonks, J.G. Bodmer, W.F. Bodmer

Tissue Antigens 54, 603-614 (1999)

2.04 Development and application of fluorescent assays for probing labile iron pools in biological systems1Z. Ioav Cabantchik2,3 and William Breuer2

Department of Biological. Chemistry, Hebrew University Jerusalem, Israel

2.05 均相荧光分析uHTS;近似蛋白酶分析

S.M Ashman, U.M. Haupts, K.J. Moore & A.J. Pope

Molecular Interactions & New Assay Technologies, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park Third

Avenue, Harlow, Essex CM19 5AW

(available only as a hardcopy)

2.06 DAPI着色后DNA容量被荧光测试细胞定型

R. Labitzke, M. Teifel and P. Friedl

Technische Hochschule Darmstadt, Institut für Biochemie, Petersenstraße 22, 64287 Darmstadt, Germany

2.07 在96-384-1536孔板中比较学习酶的分析和细胞探针分析

M. Berg, K. Undisz, R. Thiericke, S. Grabley, T. Moore* and C. Posten**

Hans-Knoelll-Institut für Naturstoff-Forschung e.V., Beutenbergstr. 11, D-07745 Jena, Germany

*Opel Jena GmbH – A Company of JENOPTIK Bioinstruments, Goeschwitzer Str. 40, D-07745 Jena, Germany

** Institut fuer Mechanische Verfahrenstechnik und Mechanik der Universität Karlsruhe (TH), Kaiserstraße 12, D-76185

Karlsruhe, Germany

(available only as a hardcopy)

2.08 Aspergillus Fumigatus 的6个分离体活性氧粒子的荧光测试

U-C. Hipler1, U. Wollina1, D. Denning2, B. Hipler3

1Department of Dermatology, Friedrich-Schiller-University, Jena, Germany

2Department of Microbiology, University of Manchester, United Kingdom

3BMG Labtechnologies GmbH, Offenburg, Germany

2.09 利用QuantaBluTM 作为过氧化酶培养基/比较 QuantaBluTM 比色过氧化酶培养基

PIERCE, 3747 N, Meridian Rd., PO Box 117, Rockford, IL 61105 U.S.A

2.10 QuantaBluTM 荧光过氧化酶底物供应迅速数据采集和高灵敏

M. Dean Savage, Thomas King-Spengler, Stephen E. Johnson*

PIERCE, 3747 N, Meridian Rd., PO Box 117, Rockford, IL 61105 U.S.A

*Community Unit School District #5 (Sterling III)

2.11The use of a conventional tissue culture plate as an optically accessible perfusion chamber for in situ assays and for long-term cultivation of mammalian cells

M.A. Freyberg and P. Friedl

Technische Hochschule Darmstadt, Inst. F. Biochemie, Petersenstr. 22, D-64287 Darmstadt, Germany

Cytotechnology 26: 49-58, (1998)

2.12PHYSIOLOGICAL AND PATHOLOGICAL PROPERTIES OF LABILE FORMS OF IRON IN BIOLOGICAL FLUIDS.

P.I.: Dr. Z. Ioav Cabantchik, MD PhD, Professor and Dr. William Breuer, Research Associate. Dept. of Biological Chemistry,

Hebrew University, Jerusalem 91904, Israel.

2.13 PicoGreen dsDNA定量试剂和Kits

Molecular Probes

Molecular Probes Product Information Revised 07-Nov-2000

2.14 Liver Mixed Function Oxidase Activity

James Ataria, Landcare, New Zealand

FLUOstar Application Note – James Balmer, June 1999

2.15 组胺分析

FLUOstar Application note – BMG Labtechnologies, Inc.

2.16 新的荧光技术平台分析高通量细胞毒素和增值分析

Magdalena Wodnicka. 1.4, Richard D. Guarino1, John J. Hemperly1, Mark R. Timmins2, David Stitt3 and J. Bruce Pitner1

1BD Technologies, Research Triangle Park, NC.

2BD BioSciences, Bedford, MA.

3BD BioSciences, Baltimore, MD.

4 Current address: Center for Thrombosis and Hemostasis, University of North Carolina at Chapel Hill, Chapel Hill. NC.

Presented as a poster at the 5th Annual conference of the Society for Biomolecular Screening. Edinburgh. Scottland. September

12-16.1999

Journal of Biomolecular Screening 5, 141-152 (2000)

2.17 油脂分析用FLUOstar Galaxy

Dr. Franz Tatzber KEG, Leopoldsgraben 38d, Klosterneuburg

Department of Traumatology, University of Graz, Austria. Renate.wildburger@kfunigraz.ac.at

2.18 用 BMG POLARstar Galaxy  PicoGreen分子探针分析

BMG Labtechnologies, Offenburg Germany

Application Note 2003, Last Revision 12/22/00

2.19 Ebselen protects mice against T cell-Dependent TNF-mediated apoptotic liver injury

Gisa Tiegs, Sabine Kusters, Gerald Kunstle, Hannes Hentze, Alexandra K. Kiemer and Albrecht Wendel

The Journal of Pharmacology and Experimental Therapeutics 287(3), 1098-1104.

2.20 Designing new metal affinity peptides by random mutagenesis of a natural metal-binding site

Markus M.Enzelberger, Stefan Minning, and Rolf D.Schid

Journal of chromatography A, 898, 83-94 (2000)

2.21Nucleo-cytoplasmic shuttling of the hdm2 oncoprotein regulates the levels of the p53 protein via a pathway used by the human immunodeficiency virus rev protein

Judith Roth, Matthia Dobbelstein, Deborah A. Freedman, Thomas Shenk and Arnold J. Levine

The EMBO Journal 17(2), 554-564 (1998)

2.22 Potential Drugs Against Cervical Cancer: Zinc-ejecting inhibitors of the human Papillomavirus Type 16 E6 Oncoprotein

Walter Beerheide, Hans-Ulrich Bernard, Yee-Joo Tan, Arasu Ganesan, William G. Rice, Anthony E. Ting

Journal of the National Cancer Institute 91(14), 1211-1220 (1999)

2.23 New Miniaturized Highly Sensitive Immunoassay device for Quantitative Measurement of Soluble or Particular Antigen or Antibodies in a Liquid Sample

Alain Rousseau and Michel Canton

Clinical Chemistry 15(9), 1685-1687 (1999)

2.24 Modulation of Multidrug Resistance Protein Expression In Porcine Brain Capillary Endothelial Cells In Vitro

HEIKE GUTMANN, MICHAEL T ¨ OR ¨ OK, GERT FRICKER, J ¨ ORG HUWYLER1, CHRISTOPH BEGLINGER, AND J ¨ URGEN DREWE

Drug Metabolism and Disposition, 27(8), 937-941 (1999)

2.25 Paradoxical Effects of a Synthetic Metalloproteinas Inhibitor That Blocks Both P55 and P75 TNF Receptor Shedding and TNFa Processing in RA Synovial Membrane Cell Cultures

Lynn M. Williams, Deena L. Gibbons, Andrew Gearing,* Ravinder N. Maini, Marc Feldmann, and Fionula M. Brennan

Journal of Clinical Investigations, 97(12), 2833-2841 (1996)

2.26 Comparative pharmacological studies of melatonin receptors: MT1, MT2 and MT3/QR2. Tissue distribution of MT3/QR2

Olivier Nosjean, Jean-Paul Nicolas, Frederique Klupsch, Philippe Delagrange, Emmanuel Canet, Jean A. Boutin

Biochemical Pharmacology 61, 1369-1379, (2001)

2.27 Top-down morphogenesis of colorectal tumors

Ie-Wing Shih, Tian-Li Wang, Giovanni Trverso, Kathy Roman, Stanley R. Hamilton, Shmuel Ben-Sasson, Kenneth W. Kinzler and Bert Vogelstein

The Proceedings of the National Academy of Science 98(5), 2640-2645 (2001)

2.28 Inactivation of a Defined Active Site in the mouse 20S proteasome Complex Enhances Major Histocompatibility Complex Class I Antigen presentation of a murine cytomegalovirus

Gunter Schmidtke

Journal of Experimental Medicine 187(10), 1641-46 (1998)

2.29 Long-wavelength iodide-sensitive fluorescent indicators for measurement of functional CFTR expression in cells

Sujatha Jayaraman, Leah Teitler, Bohdan Skalski and A.S.Verkman

American Journal of Physiology 277(Cell Physiology 46), C1008-1018 (1999)

2.30 Tightly regulated and inducible expression of rabbit CYP2E1 using a tetracycline-controlled expression system

Jian-Ya Huan and Dennis R.Koop

Drug Metabolism and Disposition 27(4), 549-554 (1999)

2.31 Detection of immunoreactive napsin A in human urine

Vesna Schauer-Vukasinovic, Hanno Langen and Thomas Giller

Biochimica et Biophysica Acta 1524, 51-56 (2000)

2.32 Aromatic carboxylic acids as farnesyl surrogates in farnesyl surrogates in farnesylpyrophosphate-based farnesyltransferase inhibitors

Martin Schlitzer and Isabel Sattler

Bioorganic & Medicinal chemistry 7, 2391-2395 (1999)

2.33 The effect of a metalloproteinase inhibitor(G15402) on tumour necrosis factor-a  (TNF-a) and TNF-a receptors during human endotoxemia

Pascale E.P.Dekkers, Fanny N. Lauw, Tessa ten Hove, Anje A.te Velde, Phillip Lumley, David Becherer, Sander J.H.van Deventer and Tom van der Poll

Blood 94(7), 2252-2258 (1999)

2.34 A continuous fluorescence Assay for the study of P-Glycoprotein-mediated Drug Efflux using inside-out membrane vesicles

Mary Bebawy, Michael B.Morris and Basil D. Roufogalis

Analytical Biochemistry 268, 270-277 (1999)

2.35 Homogeneous FRET assays for the detection for the detection of biomolecular events in a fluorescent imaging system

J.F.Wright, J.George, C.G.Norey, P.M.Baxendale and D.D Burns

Nycomed Amersham Plc, Cardiff Laboratories, Forest Farm Estate, Whitchurch, Cradiff, CF14 7YT, Wales, UK

Jane.wright@eu.apbiotech.com

2.36 Assay of acidic aspartic protease activity using pH stable fluorescent probes and fluorescence imaging

D.Y.Lee, M.J.Hatcher, J.George, C.G.Norey, P.M.Baxendale and D.D.Burns

Amersham Pharmacia Biotech UK Limited, Amersham Place, Little Chaliont, Buckinghamshire HP79NA, UK

Denise.lee@eu.apbiotech.com

2.37 Cyclic-AMP Assay kit, HitHunterTM enzyme fragment complementation technology

DiscoverX, 42501 Albrae Street, Fremont, CA 94538

2.38 Molecular fluorescence and absorbance measurements using microscale methods

CHEM4111Lexperiment E:

2.39 Screening assays for the mitochondrial permeability transition using a fluorescence multiwell plate reader

James R.Blattner, Lihua He and John J.Lemasters

Analytical Biochemistry 295, 220-226 (2001)

2.40 BRET Demo 13.3.02 Omnilab Laborzentrum  Dr. Sigrun Mink G2M Cancer Drugs AG

C/o Forschungszentrum Karlsruhe

Eggenstein-Leopoldshfen, Germany

2.41 Fluorescence and Homogeneous assays  TRACE®/HTRF®

2.42 Evidence that serpin architecture intrinsically supports papain-like cysteine protease inhibition: engineering a1-antitrypsin to inhibit cathepsin proteases

James A. Irving, Robert N. Pike, Weiwen Dai, Dieter Bro¨mme, D. Margaret Worrall, Gary A. Silverman,

Theresa H. T. Coetzer, Clive Dennison, Stephen P. Bottomley, and James C. Whisstock

Biochemistry 41, 4998-5004 (2002)

 2.43Molecular determinants of the mechanism underlying acceleration of the interaction between antithrombin and factor Xa by heparin pentasaccharide

Noelene S.Quinsey, James C. Whisstock, Bernard Le Bonniec, Virginie Louvain, Stephen P. Bottomley and Robert N. Pike

The Journal of Biological Chemistry 277(18), 15971-15978 (2002)

2.44 Inhibitory activity of a heterochromatin-associated serpin (MENT) against papain-like cysteine proteinases affects chromatin structure and blocks cell proliferation

James A. Irving, Sain S. Shushanov, Robert N. Pike, Evgenya Y. Popova, Dieter Bro¨mme, Theresa H. T. Coetzer, Stephen P. Bottomley, Iaroslava A. Boulynko, Sergei A. Grigoryev, and James C. Whisstock

The Journal of Biological Chemistry 277(15), 13192-13201 (2002)

2.45 Two-color mutation detection in 96-well microplates

Elaine S.Mansfield and Paolo Fortina

Molecular Dynamics, Application Note 54

2.46 Alkaline-mediated differential interaction (AMDI): A simple automatable single-nucleotide polymorphism assay

Sylvia Bartlett, Josef Straub, Susan Tonks, Rush Spencer Wells, Julia G.Bodmer and Walter F.Bodmer

Proceedings of the National Academy of Sciences 98(5), 2694-2697 (2001)

2.47 Top-down morphogenesis of colorectal tumors

Ie-Ming Shih, Tian-Li Wang, Giovanni Traverso, Kathy Romans, Stanley R.Hamilton, Shmuel Ben-Sasson, Kenneth W. Kinzler and Bert Vogelstein

Proceedings of the National Academy of Sciences 98(5), 2640-2645 (2001)

2.48 Assay for lactate dehydrogenase (LDH) activity (mmethod)

As per Richards et al (1975) Clinical Chemistry, 21, 1018 and Moldeus P, Hogberg J, Orrenius S. (1978) Methods Enzymol, 52, 60.

2.49 LOD, LOQ and %RSD for POLARstar Galaxy

BMG Labtechnologies Application Note 2006

2.50 The Lys1010-Lys1325 fragment of the Wilson’s disease protein binds nucleotides and interacts with the N-terminal domain of this protein in a copper-dependent manner

Ruslan Tsivkovskii, Brian C. MacArthur and Svetlana Lutsenko

The Journal of Biological Chemistry 276(3), 2234-2242 (2001)

2.51Evidence that genetic instability occurs at an early stage of colorectal tumorigensis

Ie-Ming Shih, Wei Zhou, Steven N. Goodman, Christoph Lengauer, Kenneth W. Kinzler and Bert Vogelstein

Cancer Research 61, 813-822 (2001)

2.52 The C-terminal Nuclear Localization signal of the sex-determining region Y (SYR) high mobility group domain mediates nuclear import through importin  b1

Jade K.Forwood, Vincent Harley and David A. Jans

The Journal of Biological Chemistry 276(49), 46575-46582 (2001)

2.53 Evaluation of human endothelial-like ECV304 cells and immortalized mouse brain endothelial bEnd5 cells in co-cultures with rat glioma C6 cells as in vitro models of the blood-brain barrier

Emma Alenius

2.54 Identification of the bacteria-binding peptide domain on salivary agglutinin 9gp-DMBT1), a member of the scavenger receptor-cysteine rich superfamily

Floris J. Bikker, Antoon J. M. Ligtenberg, Kamran Nazmi, Enno C. I. Veerman, Wim van ’t Hof, Jan G. M. Bolscher, Annemarie Poustka, Arie V. Nieuw Amerongen, and Jan Mollenhauer

The Journal of Biological Chemistry June 5 2002

2.55 Proteasome Inhibition in Glyoxal-treated Fibroblasts and Resistance of Glycated Glucose-6-phosphate dehydrogenase to 20 S Proteasome Degradation in Vitro*

Anne-Laure Bulteau, Philippe Verbeke, Isabelle Petropoulos, Alain-Franc¸ois Chaffotte,

and Bertrand Friguet_

The Journal of Biological Chemistry 276(49), 45662-45668 (2001)

2.56 Evidence for CPP32 activation in the absence of apoptosis during T lymphocyte stimulation

Christine Miossec, Veronique Dutilleul, Florence Fassy and Anita Diu-Hercend

The Journal of Biological Chemistry 272(21), 13459-13462

2.57 The Lys1010–Lys1325 Fragment of the Wilson’s Disease Protein Binds Nucleotides and Interacts with the N-terminal Domain of This Protein in a Copper-dependent Manner

Ruslan Tsivkovskii, Brian C. MacArthur, and Svetlana Lutsenko

The Journal of Biological Chemistry 276(3), 2234-2242 (2001)

2.58 Homogeneous fluoroimmunoassay of a pyrethroid metabolite in urine

Evgenia G. Matveeva, Guomin Shan , Ian M. Kennedy , Shirley J. Geea, Donald W. Stoutamire , Bruce D. Hammocka,

Analytical Chimica Acta 444, 103-117(2001)

2.59 4-Nitrobenylidene malononitrile reduces apoptosis-mediated liver injury in mice

Alexey Vanichkin, Miriam Patya, Irina Lagovsky, Asher Meshorer and , Abraham Novogrodsky

Journal of Hepatology 36, 631-636 (2002)

2.60 Circular dichroism spectroscopy of fluorescent proteins

Nina, V. Visser, Mark A. Hink, Jan Willem Borst, Gerard N.M. van der Krogt, Antonie J.W.G Visser

FEBS Letters 521, 31-35 (2002)

2.61 Potential problems with fluorescein diacetate assays of cell viability when testing natural products for antimicrobial activity

Joanne M. Clarke, Michael R. Gillings, Nanda Altavilla and Andrew J. Beattie

Journal of Microbiological Methods 46, 261-267 (2001)

2.62 Three cases of acquired von Willerbrand disease associated with systemic lupus erythematosus

J.F, Viallard, J.L. Pellergrin, C. Vergnes, A.Borel-Derlon, G.Clofent-Sanchez, A.T.Nurden, B.Leng, and P.Nurden

British Journal of Haematology 105, 532-537 (1999)

2.63 Peptide mimics of the vesicular stomtitis viru G-protein transmembrane segment drive membrane fusion in vitro

Dieter Langosch, Bettina Brosig and Rudiger Pipkorn

The Journal of Biological Chemistry 276(34), 32016-32021 (2001)

2.64 Determination of glucose in soft drinks using its enzymatic oxidation and the detection of formed hydrogen peroxide with dinuclear iron (III) complex

Diedrich Harms, Jorg Meyer, Lars Westerheide, Bernt Krebs and Uwe Karst

Analytica Chimica Acta 401, 83-90 (1999)

2.65 Neurons are protected from excitotoxic death by p53 antisense oligonucleotides delivered in anionic liposomes

Aparna Lakkaraju, Janet M. Dubinsky, Walter C. Low and Yueh-Erh Rahman

The Journal of Biological Chemistry 276(34), 32000-32007 (2001)

2.66 Purification and characterization of active recombinant human napsin A

Vesna Schauer-Vukasinovic, Daniel Bur, Eric Kitas, Daniel Schlatter, Gerard Rosse, Hans-Werner Lahm and Thomas Giller

European Journal of Biochemistry 267, 2573-2580 (2000)

2.67  A convenient fluorometric method for the detection of extracellular N-acetylglucosaminidase production by filamentous fungi

Carmi Omero , Benjamin A. Horwitz , Ilan Chet

Journal of Microbiological Methods 43, 165-169 (2001)

2.68 Potential use of multienobiotic defense mechanism(MXDM) in Dreissena Polymorpha as a biomarker for the monitoring of freshwater pollution

Marc Parant and Sandrine Pain

Water Research 35(15), 3743-3748 (2001)

2.69 Application of the luciferase cell culture bioassay for the detection of refined petroleum products

Michael H. Ziccardi, Ian A., Gardner, Jonna A.K. Mazet and Michael S. Denison

Marine Pollution Bulletin 2002

2.70 Fluorometric screening for metabolism – based drug – drug interactions

Charles L. Crespi and David M. Stresser

Journal of Pharmacological and Toxicological Methods 44, 325-331(2000)

2.71 Development of a simple and sensitive fluorimetric method for isolation of coumaphos-hydrolysing bacteria

R.L. Harcourt, I. Horne, T.D.Sutherland, B.D. Hammock, R.J. Russell and J.G.Oakeshott

Letters in Applied Microbiology 34, 263-268(2002)

2.72 Labile iron in parental iron formulations and its potential for generating plasma nontransferrin-bound iron in dialysis patients

B.P. Esposito, W. Breuer, I. Slotki, and Z.I.Cabantchik

European Journal of Clinical Investigation 32(1), 42-49 (2002)

2.73 Association studies between haemochromatosis gene mutations and the risk of cardiovascular diseases

G.Hete, A.Elbaz, J. Garlepy, V. Nicaud, D. Arveller, C. Morrison, F.Kee, A.Evans, A.Simon, P.Amarenco, F. Camblen and B. Grandchamp

European Journal of Clinical Investigation 31, 382-388(2001)

2.74 Development of new assays and improved procedures for the purification of recombinant human chymase

Gilles Ferry, Ludovic Gillet, Vincent Bruneau, Jesus M. Banales, Phillippe Beauverger, Francis Coge, Jean-Pierre Galizzi, Elisabeth Saclbert, Takehiro Okamoto, Hidenori Urata and Jean A. Boutin

European Journal of Biochemistry 268, 5885-5893 (2001)

2.75 Effect of primary and secondary structure of oligodeoxyribonucleotides on the fluorescent properties of conjugated dyes

Irina Nazarenko, Rick Pires, Brian Lowe, Mohamad Obaidy and Ayoub Rashtchian

Nucleic Acid Research 30(9), 2089-2195 (2002)

2.76 Characterization of Streptococcus pneumoniae 5-enolpyruvylshikimate 3- phosphate synthase and its activation by univalent cations

Wensheng Du, Nicola G. Wallis, Marie J. Mazzulla, Alison F. Chalker, Lily Zhang, Wu-Schyong Liu,

Howard Kallender and David J. Payne

European Journal of Biochemistry 267, 222-227 (2000)

2.77 Quantitative in vivo measurement of glutathione in Arabidopsis cells

Andreas J. Meyer, Mike. J. May and Mark Fricker

The Plant Journal 27(1), 67-78 (2001)

2.78 Green fluorescent protein-based halide indicators with improved chloride and iodide

Luis J.V. Galietta, Peter M. Haggie and A.S. Verkman

FEBS Letters 499, 220-224 (2001)

2.79 A microplate reader-based nonisotropic histone deacetylase activity assay

Birgit Heltweg and Manfred Jung

Analytical Biochemistry 302, 175-183(2002) 

3. Time-Resolved Fluorescence (TR-F) / TRET ApplicationsTRF and TRF-FRET分析原理请查看链接

3.01 Time-Resolved Fluorescence Energy Transfer and Scintillation Proximity DNA Helicase Assays for HTP Screening

David L. Earnshaw, Keith J. Moore, Hakim Djaballah, Anthony J. Jurewicz, Kenneth J. Murray & Andrew J. Pope

Molecular Screening Technologies, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Third Avenue, Harlow,

Essex CM19 5AW, U.K.

(available only as a hardcopy)

3.02 Investigations on the Enzyme Catalysed Oxidation of Peroxidase Substrates Fluorimetric Determination of Hydrogen Peroxide by Peroxidase Enhanced Lanthanide Luminescence

Jörg Meyer and Uwe Karst

Westfälische Wilhelms-Universität Münster, Analytische Chemie, Wilhelm-Klemm-Str. 8, D-48149 Münster

  4. Cell assays Applications

4.01 Rapid Measurements of Intracellular Calcium Using a Fluorescence Plate Reader

Kedan Lin, Wolfgang Sadée and J. Mark Quillan

University of California, San Francisco, CA, USA

Biotechniques 26, 318-326 (1999)

4.02 Recording of Intracellular Ca+, CI-, pH and Membrane Potential in Cultured Astrocytes using a Fluorescence Plate

Reader

Timothy J. Manning Jr. and Harald Sontheimer

Department of Neurobiology, University of Alabama at Birmingham

Journal of Neuroscience Methods (1999)

4.03 Intracellular calcium measurement using the cell permanent Fluo-3 acetomethyl (AM) ester

Hwei Ling Ong, Mario Froscio and Gregory J. Barritt

Flinders University School of Medicine, Bedford Park, South Australia, 5042

FLUOstar Application note – James Balmer, July 1998

4.04Intracellular Calcium Assay performed on NOVOstar Microplate Reader

Anders heding, Novo Nordisk, New Lead Discovery, Novo Alle’, 2880 bagsvaerd, Denmark, AHED@novonordisk.com

NOVOstar Application note – BMG Labtechnologies

4.05 Intracellular calcium measurements with Fluo3

Hwei Ling Ong, Mario Froscio and Gregorz J. Barritt

Department of Medical Biochemistry, Flinders University School of Medicine, Bedford Park, South Australia, 5042

4.06 New BCECF pH Indicator – A Proven Method for Measuring Intracellular pH

James Balmer

BMG Labtechnologies, Australia

4.07 Rapid functional screening at dopamine receptors by measuring intracellular calcium with the NOVOstar fluorescence reader

Barbara Hoefgen and Matthias U.Kassack

University of Bonn, Pharmaceutical Institute, An der Immenberg 4, 53121 Bonn, Germany, kassack@uni-bonn.de

BMG Labtechnologies, Application Note 107

4.08 pABC11 (Also known as MOAT-C and MRP5), a member of the ABC family of proteins, has anion transporter activity but does not confer multidrug resistance when over expressed in human embryonic kidney 293 cells

Marcia A.McAleer, Maria A.Breen, Nina L. White and Nicholas Matthews

The Journal of Biological Sciences, 274(13), 23541-23548 (1999)

4.09 Inhibition of human caspases by peptide-based and macromolecular inhibitors

Margarita Garcia-Calvo, Erin P.Peterson, Barbara Leiting, Rejean Rue, Donald W. Nicholson and Nancy A.Thornberry

The Journal of Biological Chemistry, 273(49), 32608-32613 (1998)

4.10 Dual role of caspase-11 in mediating activation of caspase-1 and caspase-3 under pathological conditions

Shin-Jung Kang, Suyue Wang, Hideaki Hara, Erin P.Peterson, Shobu Namura, Sepideh Amin-Hanjani, Zhihong Huang, Anu Srinivasan, Kevin J. Tomaselli, Nancy A.Thornberry, Michael A. Moskowitz and Junying Yuan

The Journal of Cell Biology, 149(3), 613-622 (2000)

4.11 A combinatorial approach defines specificities of members of the caspase family and Granzyme B – Functional relationships established for key mediators of apoptosis

Nancy A. Thornberry, Thomas A. Rano, Erin P. Peterson, Dita M. Rasperi, Tracy Timkey, Margarita Garcia-Calvo, Vicky M. Houtzageri, Penny A. Nordstromi, Sophie Royi, John P. Vaillancourti, Kevin T. Chapman, and Donald W. Nicholsoni

The Journal of Biological Chemistry 272(18), 17907-17911 (1997)

4.12 Aminooxypentane addition to the  chemokine macrophage inflammatory protein-1aP increases receptor affinities and HIV inhibition

Jane R. Townson, Gerard J. Graham, Nathaniel R. Landau, Beth Rasalai, and

Robert J. B. Nibbs

The Journal of Biological Chemistry 275(50), 39254-39261 (2000)

4.13 Novel CFTR chloride channel activators identified by screening of combinatorial libraries based on flavone and benzoquinolizinium lead compounds

Luis V.J. Galietta, Mark F. Springsteel, Masahiro Eda, Edmund J. Niedzinski, Kolbot By, M. J. Haddadin, Mark J. Kurth, Michael H. Nantz and A.S. Verkman

The Journal of Biological Chemistry (2001)

4.14 Modulation of glioma cell migration and invasion using Cll- and K+ ion channel blockers

Liliana Soroceanu, Timothy J.Manning Jr and Harald Sontheimer

The Journal of Neurosciences 19(14), 5942-5954 (1999)

4.15 Evidence for CPP32 activation in the absence of apoptosis during T lymphocyte stimulation

Christine Miossec, Veronique Dutilleul, Florence Fassy and Anita Diu-Hercend

The Journal of Biological Chemistry 272(21), 13459-13462 (1997)

4.16 Definition and redesign of the extended substrate specificity of granzyme B

Jennifer L. Harris, Erin P. Peterson, Dorothy Hudig, Nancy A. Thornberry and Charles S. Craik

The Journal of Biological Chemistry 273(42), 27364-27373 (1998)

4.17Hyperosmolality causes growth arrest of murine kidney cells – induction of GADD45 and GADD153 by osmosensing via stress-activated protein kinase 2

Dietmar Kultz, Samira Madhany and Maurice B. Burg

The Journal of Biological hemistry 273(22), 13645-13651 (1998)

4.18 Identification of the melatonin-binding site MT3 as the quinone reductase 2

Olivier Nosjean, Myriam Ferro, Francis Coge´, Philippe Beauverger, Jean-Michel Henlin, Franc¸ois Lefouloni, Jean-Luc Fauche`re, Philippe Delagrange, Emmanuel Cane, and Jean A. Boutin

The Journal of Biological Chemistry 275(40), 31311-31317 (2000)

4.19 pH-dependent changes of nitric oxide, peroxynitrite, and reactive oxygen species in hepatocellular damage

Zhijun Shu, Martin Jung, Hans-G. Beger, Michael Marzinzig, Fuli Han, Urwe Butzer, Uwe B. Bruckner, And Andreas K. Nussler

American Journal Physiology 273, G1118-G1126(1997)

4.20  Rapid functional screening at dopamine receptors by measuring intracellular calcium with the BMG NOVOstar fluorescence microplate reader

Barbara Hoefgen and Matthias U.Kassack  BMG Labtechnologies Application Note

4.21Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2’,7’-dichlorodihyrofluorescein diacetate, 5(and)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123

Stephen L. Hempel, Garry R. Buettner, Yunxia Q.O’Malley, Duane A. Wessels and Dawn M. Flaherty

Free Radical Biology & Medicine 27(1/2), 146-159 (1999)

4.22Application of yeast cells transformed with GFP expression constructs containing the RAD54 or RNR2 promoter as a test for the genotoxic potential of chemical substances

Victor Afanassiev, Mark Sefton, Thaddao Anantachaiyong, Gordon Barker, Richard Walmsley and Stefan Wolfl

Mutation Research 464, 297-308 (2000)

4.23 Design of a fluorescence microplate reader based assay for the assessment of calcium efflux pathways: implications for the screening of novel compounds

Nerida S. Jenkins, Nicola A. Holman, Matthew G. Seymour and Gregory R. Monteith Poster

4.24 MTT (dimethylthiazol-diphenyltetrazolium bromide) cytotoxicity assay Method

4.25 Neutral Red cytotoxicity assay Method

4.26 Monitoring adherent cell proliferation on BD oxygen Biosensor Method

4.27 Uptake, recycling and antioxidant actions of a-Lipoic acid in endothelial cells

Wright Jones, Xia Li, Zi-Chao Qu, Laureta Parriott, Richard R. Whitesell and James M.May

Free Radical Biology & Medicine 33(1), 83-93 (2002)

4.28 Effect of glutathione depletion on antitumor drug toxicity (apoptosis and necrosis) in U-937 human promoncytic cells : the role of intracellular oxidation

Alfonso Troyano, carlos Fernandez, patricia Sancho, Elena de Blas and Patricio Aller

The Journal of Biological Chemistry 276(50), 47107-47115(2001)

4.29 b-Bungatoxin is a potent inducer of apoptosis in cultured rat neurons by receptor-mediated internalization

Mathias Herkert, Olena Shakhman, Elisabeth Schweins and Cord-Michael Becker

European Journal of Neuroscience 14, 821-828 (2001)

4.30 Bacterial surface association of heat-labile enterotoxin through lipopolysaccharide after secretion via the general secretory pathway

Amanda L. Hortsman and Meta J. Kuehn

The Journal of Biological Chemistry June 26 2002

4.31 Calmodulin Binding to G protein-coupling domain of opiod receptors

Danxin Wang, Wolfgang Sadee and J. mark Quillan

The Journal of Biological Chemistry 274(31), 22081-22088 (1999)

4.32 Expression of functional chemokine receptors CXCR3 and CXCR4 on human melanoma cells

Mar M. Robledo, Rube´n A. Bartolome, Natividad Long,Jose´ Miguel Rodrý´guez-Frade, Mario Mellado, Isabel Longo, Goos N. P. van Muijen, Paloma Sa´nchez-Mateo, and Joaquin Teixido

The Journal of Biological Chemistry 276(48), 45098-45105 (2001)

4.33 Chaperone suppression of cellular toxicity of Huntington is independent of polyglutamine aggregation

Hui Zhou, Shi-Hua and Xiao-Jiang Li

The Journal of Biological Chemistry 276(51), 48417-48424(2001)

4.34 Human immunodeficiency virus type 1 replication inhibition by the bidentate iron chelators CP502 and CP511 is caused by proliferation inhibition and the onset of apoptosis

N. A. Georgiou, T. van der Bruggen, M. Oudshoorn, R. C. Hider, J. J. M. Marx and B. S. van Asbeck

European Journal of Clinical Investigation 32(1), 91-96 (2002)

4.35 Impairment of proteasome function upon UVA- and UVB- irradiation of human keratinocytes

Anne-Laure Bulteau, Marielle Moreau, Carine Nizard and Bertrand Friguet

Free Radical Biology & Medicine 32(11), 1157-1170 (2002)

4.36 Reversible protein kinase C activation in PC12 cells: effect of NGF treatment

Jean-Luc Dupont, Agnes Janshazi, Mohamed Bellahcene, Serge Mykita and Jean de Barry

European Journal of Neuroscience 12, 25-226 (2000)

4.37 Nuclear Ca2+ calmodulin translocation activated by m-Opoid (OP3) receptor

Danxin Wang, Lara M. Tolbert, Kurt W. Carlson and Wolfgang Sadee

Journal of Neurochemistry 74, 1418-1425 (2000)

4.38 Investigation of the cellular uptake of E-Selectin-targeted immunoliposomes by activated human endothelial cells

Stephen Kessner, Annett Krause, Ulrich Rothe and Frd Bendas

Biochimica et Biophysica Acta 1514, 177-190 (2001)     

4.39 Integrins of the b1 family influence keratinocyte-lymphocyte interaction

Mailka Boukhelifa, Yolene Paulin, Jacqueline Font, Jacqueline Pichon, Mirelle Giner, Josiane Wantyghem, Michele Aubery and Braut-Boucher

Journal of Investigative Dermatology 111, 650-655 (1998)

4.40 Therapeutic effects of cysteine protease inhibition in allergic lung inflammation: inhibition of allergen-specific Y lymphocyte migration

G.T. Layton, S.J. Harris, F.A. Bland, S.R. Lee, S. Fearn, J.Kaleta, M.L.Wood, A.Bond and G.Ward

Inflammation Research 50, 400-408(2001)

4.41 Novel CFTR chloride channel activators identified by screening of combinatorial libraries based on flavone and benzoquinolizinium lead compounds

Luis J.V. Galietta, Mark F. Springteel, Masahiro Eda, Edmund J. Niedzinski, Kolbot By, M.J. Haddadin, Mark J. Kurth, Michael H. Nantz and A.S Verkman

The Journal of Biological Chemistry 276(23), 19723-19728 (2001

5. Luminescence ApplicationsL发光分析的原理请查看链接

5.01 A Low Concentration of Ethanol Reduces the Chemiluminescence of Human Granulocytes and Monocytes but Not the Tumor Necrosis Factor Alpha Production by Monocytes after Endotoxin Stimulation

Alexandr Parlesak 1*, Jens P. Diedrich 1, Christian Schäfer 2 and Christiane Bode1

Department of Physiology of Nutrition, Hohen heim University, 1 and Department of Internal Medicine I (Gastroenterology and Hepatology), Robert-Bosch-Krankenhaus, 2 Stuttgart, Germany

Infection and Immunity 66, 2809-2813 (1998)

5.02 Inhibitory Effect of Terbinafine on Reactive Oxygen Species Generated By Candida Albicans

U.-Ch. Hipler, Ch. Schröter, U. Wollina, B. Hipler1

Department of Dermatology, Friedrich-Schiller-Universität Jena, Germany

1BMG Labtechnologies GmbH, Offenburg, Germany

5.03 Development of a rapid Bioluminescence Detection Technique for High-Throughput Screening of Biologically Active Samples

Alys Peisley*, Bruce G. Livett, Robert J. Capon* and John G. Down

Departments of Biochemistry and Molecular Biology

*Chemistry, University of Melbourne, Parkville, Victoria 3052, Austria

5.04 A Nonradioactive Assay for Ribosome-Inactivating Proteins

Martin Langer, Markus Rothe, Jürgen Eck, Babette Möckel and Holger Zinke1

Analytical Biochemistry 243, 150-153 (1996), Article no. 0493

5.05 Manual for Chemiluminescence testing of antibody and effector cells

Morten Dziegiel

National University Hospital Copenhagen, Department of Clinical Immunology, Blegdamsvej 9, DK-2100 Copenhagen

5.06 Generation of Reactive Oxygen Species (ROS) and their Measurement by Means of Chemiluminescence

Bernd Hipler

LUMIstar Application note – BMG Labtechnologies

5.07 The use of an ATP Bioluminescence Assay to Quantify Cell Cytotoxicity

Bernd Hippler

LUMIstar Application note – BMG Labtechnologies

5.08 Assessment of BMG LUMIstar Microplate luminometer for screening seedling mutations

Dr. M. R. Knight

Oxford University, Dept. of Plant Science

5.09 Cap-independent translational enhancement of turnip crinkle virus genomic and subgenomic RNAs

Feng Qu and T. jack Morris

Journal of Virology 74(3), 1085-1093(2000)

5.10 Covalent modification of p73a by SUMO-1: Two hybrid screening with p73 identifies novel sumo-1-interacting proteins and sumo-1 interaction motif

Adrian Minty, Xavier Dumont, Mourad Kaghad and Daniel Caput

The Journal of Biological Chemistry 275(46), 36316-36323 (2000)

5.11Sensitivity of Human immunodeficiency virus Type 1 to the fusion inhibitor T-20 is modulated by coreceptor specificity defined by the V3 loop of gp120

Cynthia A. Derdeyn, Julie M. Decker, Jeffrey N. Sfakianos, Xiaoyun Wu, William A, O’Brien, Lee Ratner, John C. Kappes, George M. Shaw, and Eric Hunter

Journal of Virology 74(18), 8358-8367(2000)

5.12Detection of 4-chlorobenoate using immobilized recombinant Escherichia coli reporter strains

Sabine Kohler, Till T. Bachmann, Jutta Schmitt, Shmshon Belkin and Rolf D. Schmid

Sensors and Actuators B 70, 139-144 (2000)

5.13  ABEL® anitoxidant test kit with pholasin® for peroxynitrite

ABEL-41M2

Knight Scientific Limited 22 August

5.14 ABEL® anitoxidant test kit for superoxide and other free radicals with pholasin

Microplate Luminometer Kit

ABEL-21M2

Knight Scientific Limited

5.15Monitoring Bioactivity with the light-emitting protein Pholasin®

Jan Knight

Knight Scientific Limited

5.16 Rapid, simple and sensitive blood biocompatibility tests with the light-emitting protein pholasin®

Jan Knight

Knight Scientific Limited

5.17 ABEL® cell activation test kit for whole blood or isolated cells with pholasin®  and adhuvant-KTM

Microplate Luminometer Kit

ABEL-04M

Knight Scientific Limited

5.18 ABEL® cell activation test kit for isolated cells with pholasin®  and adhuvant-KTM

Microplate Luminometer Kit

ABEL-14M

Knight Scientific Limited

5.19 ABEL® antioxidant test kit for halogenated oxidants with pholasin® Microplate Test Kit

ABEL-31M

Knight Scientific Limited

5.20 ABEL® antioxidant test kits with pholasin®

Knight Scientific Limited

5.21 ABEL® cell activated test kits with pholasin®

Knight Scientific Limited

5.22 Dual-Luciferase reporter assay system certification on the LUMIstar Galaxy (DLReady)

BMG Labtechnologies Application Note 2007

5.23 Measurement of steroids in saliva- chemiluminescence, glow luminescence and LIA’s of IBL-Hamburg

5.24 Shine a brighter light on cell viability: cell titer-Glo assay, flexible luminescent cell viability assay

Randy Hoffman, Natalie Betz and Michael Bjerke

Promega

5.25 Involvement of activator protein 1 complexes in the epithelium-specific activation of the laminin g2-chain gene promoter by hepatocyte growth factor (scatter factor)

Jorgen Olsen, Olivier Lefebvre, Christine Fritsch, Jesper T. Troelsen, Veronique Orian-Rousseau, Michele Kedinger and Patricia Simon-Assmann

Biochemical Journal 347, 407-417 (2000)

5.26 Monitoring of phosphorus bioavailability in water by an immobilized luminescent cyanobacterial reporter strain

Pat P.Y. Schreiter, Osnat Gillor, Anton Post, Shimshon Belkin, Rolf D. Schmid and Till T. Bachman

Biosensors & Bioelectronics 16, 811-818 (2001)

5.27 ABEL anitoxidant test kit with pholasin for vitamin C type antioxidants performed on LUMIstar Galaxy microplate luminometer

Bernd Hipler and Jan Knight

BMG Labtechnologies, Application Note 108

5.28 RT-PCR and chemiluminescence ELISA for detection of enteroviruses

G.E. greening, L.Woodfield,  and G.D.Lewis

Journal of Virological Methods 82, 157-166 (1999)

5.29 Biophysical and lipofection studies of DOTAP analogs

Anne E. Regelin, Stefan Fankhaenel, Laura Gurtesch, Claudia Prinz, Gunter von Kiedrowski and Ulrich Massing

Biochimica et Biophysica Acta 1464, 151-164 (2000)

5.30 The human PER1 gene is transcriptionally regulated by multiple signaling pathways

Dirk Motzkus, Erik Maronde, Uwe Grunenberg, Cheng Chi Lee, Wolf-Georg Forssmann and Urs Albrecht

FEBS Letter 486, 315-319 (2000)

5.31 Monitoring bioactivity with the light-emitting protein Pholasin®

Jan Knight

Paper presented at Medtec Ireland Conference September 2001, Galway, Ireland

5.32 Dependence of selective gene activation on the androgen receptor NH2- and carboxyl-terminal interaction

Bin He, Lori W. Lee, John T. Minges and Elizabeth M. Wilson

The Journal of Biological Chemistry May8 2002

 5.33 Determination of umbilical cord and maternal plasma concentrations of fentanyl by using novel spectophotometric and chemiluminescence enzyme immunoassays

Yannis Dotsikas, Yannis L. Loukas and Ioanna Siafaka

Analytica Chimica Acta 459, 177-185 (2002)

5.34 Inflammatory activation of neutrophils by Helicobacter pylori; a mechanism insensitive to pertussis toxin

P.S. Hansen, P.H.Madsen, S.B. Petersen and H. Nielsen

Clinical and Experimental Immunology 123, 73-80 (2001)

5.35 A tobacco matrix attachment region reduces the loss of transgene expression in the progeny of transgenic tobacco plants

Bekir Ulker, George C. Allen, William F. Thompson, Steven Spiker and Arthur K. Weissinger

The Plant Journal 18(3), 253-263 (1999)

5.36A fish cell surface receptor defined by a mAb mediates leukocyte aggregation and deactivation

V.Mulero, P.Pelegrin, M.P. Sepulcre, J.Meseguer

Developmental and comparative Immunology 25, 619-627 (2001)

5.37 Modification of the cell based assay for brevetoxins using human cardiac voltage dependent sodium channels expressed in HEK-293 cells

Elizabeth R. Fairey, Marie-Yasmine Bottein Dechraoui, Michael F. Sheets and John S. Ramsdell

Biosensors & Bioelectronics 16, 579-586 (2001)

 6. Nephelometry Applications(浊度的分析检测原理请查看链接

6.01 A High-Throughput Screening Method for the Determination of Aqueous Drug Solubility Using Laser Nephelometry in Microtiter Plates

Christopher D. Bevan, and Richard S. Lloyd*

Physical Sciences, GlaxoWellcome Research and Development, Gunnels Wood Road, Stevenage, Hertfordshire, SG1 2NY U.K. *Pharmaceutical Sciences, GlaxoWellcome Research and Development, Gunnels Wood Road, Stevenage, Hertfordshire, SG1 2NY U.K.

Anal. Chem. 72, 1781-1787 (2000)

6.02 High throughput aqueous drug solubility by laser nephelometry

Christopher D. Bevan and Richard S. Lloyd

GlaxoWellcome Research and Development

(available only as a hardcopy)

6.03 Automating the Determination of Aqueous Drug Solubility using Laser Nephelometry in Microtitre Plates

David Proudlock*, Malcom Willson, Barbara Carey

Glaxo Wellcome R&D Medicines Research Centre, Stevenage UK

(available only as a hardcopy)

6.04 IgG1 quantification of milk using the NEPHELOstar

Dr. R. G. Colling; AgResearch; Private Bag 3123; Hamilton; New Zealand

6.05 Information Sheet on the Nephelometric Test for Determination of Colostrum Quality

1Food Science, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton New Zealand

2 BMG Labtechnologies Pty. Ltd., PO Box 469, Mount Eliza, 3930, Victoria, Australia

6.06 Preliminary Evaluation of the BMG Nephelostar microtitre well nephelometer for solubility estimation

Chris Bevan1 and Richard Lloyd2

1Physical Sciences, 2Pharmaceutical Sciences, GlaxoWellcome Research and Development, Gunnels Wood Road, Stevenage,

Hertfordshire, SG1 2NY U.K.

6.07 Measurement of nine human milk proteins by nephelometric immunoassays: application to the determination of mature milk protein profile

Paul M.Montagne, Virginie S. Tregoat, Marie L. Cuilliere, Marie C. Bene, and Gilbert C. Faure

Clinical Biochemistry 33(3), 181-186 (2000)

6.08 Effect of Alternative Use of EDA anticoagulants on Red blood cell indices by moving average and intra-individual variation

Masae Asanuma, Hiroko Uesaka, Makoto Miyazaki, Hisaaki Hosokawa

ISLH XIVth International SYmposium

6.09 Rapid monitoring of recombinant protein products: a comparison of current technologies

Kym N. Baker, Mark H.Rendell, Ashvin Patel, Paul Boyd, Mike Hoare, Robert B. Freedman and David C. James

Trends in Biotechnology 20(4), 149-156(2000)

 7. Absorbance Applications (ABS吸收 原理)

7.01 UV Absorbance Measurements of DNA in Microplates

Evelyn L. McGown

Molecular Devices Corporation Sunnyvale, CA, USA

BioTechniques 28, 60-64 (2000)

7.02 DNA Quantification (Absorbance Mode)

BMG Labtechnologies, Germany

7.03 Determination of umbilical cord and maternal plasma concentrations of fentanyl by using novel spectophotometric and chemiluminescence enzyme immunoassays

Yannis Dotsikas, Yannis L. Loukas and Ioanna Siafaka

Analytica Chimica Acta 459, 177-185 (2002)

7.04 Regulated and unregulated mitochondrial permeability pores: a new paradigm of pore of pore structure and function?

Lihua He, John J. Lemasters

FEBS Letters 512, 1-7 (2002)

8. Other Applications

8.01 A Method for Evaluation and Comparison of Performance of Microtitre Plate Readers for uHTS

Sandra Turconi1, Martin Ruediger1, Anthony J Jurewicz2, Ricardo Macarron2, Maite De Los Frailes3, Kerry Shea1, Brian C.

Bond4, Ryan P. Bingham1, Robert P. Hertzberg2 & Andrew J. Pope1

SmithKline Beecham Pharmaceuticals, Departments of 1Molecular Interactions and Novel Assay Technologies, New Frontiers

Science Park, Harlow CM19 5AW,UK, 2Screening Sciences-US, 3Screening Sciences-Spain, Parque Tecnologico de Madrid,

28760 Tres Cantos, Spain. And 4Statistical Sciences, New Frontiers Science Park, Harlow CM19 5AW, UK

(available only as a hardcopy)

8.02 Homogeneous Fluorescence Assays for uHTS; Binding assays for 7-TM receptors using Fluorescence Intensity

Distribution Analysis (FIDA) and Fluorescence Anisotropy (FA)

Martin Rüdiger, Ulrich Haupts, Keith J Moore & Andrew J Pope

Molecular Interactions & New Assay Technologies, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park Third

Avenue, Essex CM19 5AW

(available only as a hardcopy)

8.03 Homogeneous fluorescence assays for uHTS: nucleic acid replication proteins

DL Earnshaw*, CJ Greenwood, M Ruediger and AJ Pope

Molecular Interactions & New Assay Technologies, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Third Avenue, Harlow, Essex CM19 5AW, UK

(available only as a hardcopy)

8.04 Simple absorbance based assays in 1536-well format

Patrick Lavery, Murray J B Brown & Andrew J Pope

Molecular Interactions & New Assay Technologies, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park Third Avenue, Harlow, Essex CM19 5AW

(available only as a hardcopy)

8.05 Homogeneous fluorescence readouts for miniaturized high-throughput screening: theory and practice

Andrew J. Pope, Ulrich M. Haupts and Keith J. Moore*

Molecular Interactions and New Assay Technologies, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Third Avenue, Harlow, UK CM19 5AW

*Lead Discovery Unit, GlaxoWellcome, Research and Development, Gunnels Wood Road, Stevenage, Hertfordshire, UK SG1 2NY

DDT 4, 350-362 (1999)

8.06 FlashPlate Scintillation Proximity assays for HTS; nucleic acid replication proteins

D.L. Earnshaw* & A.J. Pope

Molecular Interactions & New Assay Technologies, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Third Avenue, Harlow, Essex CM19 5AW

(available only as a hardcopy)

8.07 Important facts of the SPR-biosensor technique

Dr. Silke Angersbach; BMG Labtechnologies, Germany

8.08 Miniaturized HTS technologies- uHTS

Julian Wolcke and Dirk Ullman

Drug, Discovery Today 6(12), 637-646(2001)

 9. PCR

9.01 The role of AHA motifs in the activator function of tomato heat stress transcription factors HsfA1 and HsfA2

Pascal Doring, Eckardt Treuter, Catherine Kistner, Ruth Lyck, Alexander Chen and Lutz Nover

The Plant Cell 12, 265-278 (2000)

9.02 Protein phosphatase inhibition assay adapted for determination of total DSP in contaminated mussels

Douglas O. Mountfort, Toshiyuki Suzuki and Penny Truman

10.Filter Selection

10.01 Quick Reference Filter Guide 2001 BMG Labtechnologies

10.02Quick Reference Guide – BMG USA BMG Labtechnologies

10.03 Common Fluorophores BMG Labtechnologies

10.04Filter selection macro BMG Labtechnologies

10.05 Filter Packages BMG Labtechnologies

10.06 Excitation and emission wavelengths for Europium, samarium and Terbium

10.07BMG standard filter table

BMG Labtechnologies

11. Toxicology

11.01A high throughput screen to identify inhibitors of aromatase (CYP19)

David M. Stresser, Stephanie D. Turner, Joanne McNamara, Penny J. Stocker, Vaughn P. Miller, Charles L. Crespi and Christopher J. Patten

BD Biosciences Poster

11.02Substrate dependent effects on CYP3A4 inhibition or activation by 20 test compounds

DM Stresser, AP Blanchard, V.P. Miller and Charles L. Crespi

GENTEST Corporation

11.03Cancerostatic octadecylpiperidinoylphosphate liposomes: effect of composition on uptake by and toxicity to J774 mouse macrophage cells and MT1 breast cancer cells in vitro

Reinhard Zeisig, Renate Stahn, Anne-Dorothee Teppke and Dietrich Arndt

Anti-Cancer Drug Design 16, 19-26 (2001)

 12. Animals

12.01A fluorescence polarization assay for the detection of antibodies to Mycobacterium bovis in cattle sera

Om P. Surujballi, Anna Romanowska, Edward A. Sugden, Calude Turcotte and Michael E. Jolley

Veterinary Microbiology 87, 149-157

 
荧光趋激化(FP检测)在分子生物中的应用 2010年08月17日
使用POLARStar 做同时双发射检测,快速动力学BRET分析 2010年08月17日
使用FLUOStar OPTIMA 仪器做BRET检测 2010年08月17日
JUN-air吸附式干燥系统的工作原理 2010年08月09日
怎样选择一款多功能酶标仪,多功能酶标仪的用途 2010年08月05日
怎样选择一款适合自己使用的振荡器 2010年08月05日
BRET(Bioluminescence Resonance Energy Transfer)介绍 2009年03月26日
FRET (Fluorescence Resonance Energy Transfer )介绍 2009年03月25日
MICROTHERM型隔热材料 直径 超细二氧化硅颗粒 加压成型工艺 玻璃窑 铝熔化保温炉最小颗粒直径由7纳米(nm)构成的球状极其超细二氧化硅颗粒为主要成分,再混合微粉状辐射热遮蔽材料和增加强度用无机纤维状物质,经加压成型制作的MICROTHERM(商品名)型高性能隔热材料的性能。探讨了这种隔热材料在民用蓄热取暖设备、工业用渗碳炉、烧结等热处理炉、玻璃窑、铝熔化保温炉、钢铁、水泥、石油化工、原子能发电、燃料电池、特殊用飞行自动记录仪、防火墙等方面的应用及其使用效果。 2009年01月14日
Filters(滤光片技术) 2009年01月13日
Well Scanning in Plate Readers(孔的扫描技术) 2009年01月12日
Spectrometer(光谱技术) 2009年01月11日
Tandem Technology 2009年01月10日
Reagent Injectors (试剂进样) 2009年01月09日
Path Length Correction(光路径的修正技术) 2009年01月08日
Dual Emission Detection(双发射技术) 2009年01月07日
Dedicated Luminescence测试(DLR测试技术) 2009年01月06日
AlphaScreen® 测试(Amplified Luminescent Proximity Homogeneous Assay®) 2009年01月06日
新的MARS专用评估软件。主要使用于NEPHELOstar, OPTIMA系列和OMEGA系列和PHERAstar,NOVOstar等的微孔板仪器,增加了计算EC50, IC50, r2, 和CV 的值 2008年04月01日
Omega 系列仪器已通过DLReady™测试 2008年04月01日
用POLARStar Omega 快速检测DNA 2008年03月04日
BMGLABTECH 仪器滤光片选择指导 2008年01月11日
CSBIO GMP 证书 2006年11月20日
BOCFMOC保护氨基酸与M树脂和王氏树脂结合率的比较 2006年11月14日
用不同的微孔板做高效的时间分辨荧光分析(HTRF)研究分析可以得出不同的效果 2006年11月15日
在不同的实验分析中如何选择使用不同的微孔板  2006年11月19日
组合化学,平行合成,固相合成 2006年10月10日






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本页更新日期2019年04月20日 星期六 21:57